ISOLATION AND CHARACTERIZATION OF BETULINIC ACID AND STIGMASTEROL FROM Combretum hypopilinum STEMBARK EXTRACT
HUSSAEN ADEWUMI ISMAIL*, SADAM ADETUNJI ADEBAYO, HALIMATU SADIAT HASSAN,
ILYAS MUHAMMAD
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ahmadu Bello
University, Zaria, Nigeria.
Afr. J Pharm Res Dev; Volume 13(3): 198-209 (2021)
ABSTRACT
A natural product is a chemical compound or substance produced by living organism and have played a central role in the development of the field of organic chemistry by providing challenging synthetic targets, within the field of organic chemistry, the aim is to carry out phytochemical studies of the methanol stembark extract of Combretum hypopilinum and to isolate and establish the structure of the isolated compounds using spectroscopic technique. The stembark of the plant was air-dried under shade and pounded to coarse powdered. The powdered stembark (1kg) was extracted with 80% methanol using cold maceration method for 9 days with occasional shaking (for effective extraction). The solvent was removed using rotary evaporator and percentage yield was calculated. The extract was partitioned with n-hexane, chloroform, ethyl acetate and acetone in order of polarity. The methanol extract and partitioned fractions were subjected to preliminary phytochemical screening and the both (extract and partitions) revealed the presence of steroids, alkaloids, glycosides, terpenes, saponins, phenols, flavonoids and tannins. The n-hexane fraction was mounted on column chromatography for the isolation of compounds EA and RA furthermore, structural elucidation of isolated compounds was carried out using Infrared and Nuclear magnetic resonance and comparison with literature data. The isolated compounds were confirmed to be stigmasterol and betulinic acid.
Email of correspondence: ismaila8018@gmail.com
KEYWORDS: hypopilinum; Phytochemical screening; Chromatographic analysis; Isolation;
Stigmasterol; Betulinic acid.