PROTEA MADIENSIS OLIVER (PROTEACEAE) ROOT EXTRACT AND FRACTIONS IN INFLAMMATION AND PAIN MANAGEMENT IN MICE
UWEMEDIMO FRANCIS UMOH1*, PAUL SUNDAY THOMAS1, ARIASIKE CHIDIEBERE JOSEPH1, OLUGBENGA ANTHONY OJO2, SIFON EPHRAIM AKPAN1, EMEMOBONG INIUNAM INIUNAM1
- Department of Pharmacognosy and Natural Medicine, Faculty of Pharmacy, University of Uyo, Uyo, Ibom State, Nigeria.
- Department of Pharmacognosy, Faculty of Pharmacy, Madonna University, Elele, Rivers State, Nigeria.
Afr. J Pharm Res Dev; Volume 17(1): 38-43 ; 2025
ABSTRACT
Protea madiensis root, was studied for anti-inflammatory and analgesic properties to verify its local applications. After the collection of whole plants, roots were separated, washed and shade dried. The pulverized root was cold macerated with ethanol (70%) for 72 h, filtered and concentrated in vacuo at 40ºC. A portion of the extract was introduced into a separating funnel and successively partitioned with dichloromethane, ethyl acetate and butanol to obtain partitioned fractions (dichloromethane, ethyl acetate and butanol fractions). The remaining ethanol extract was screened for secondary metabolites. Both the ethanol extract and fractions were studied for anti-odematous and analgesic effects in mice using fresh egg albumin-induced paw oedema, xylene- induced oedema, acetic acid-induced writhing, formalin-induced paw licking and thermal-induced pain models. Data obtained revealed ethanol root extract of P. madiensis as being able to display dose-dependent anti-inflammatory effect, reduced pains caused by acetic acid, formalin and hot plate effects. All the fractions displayed potent significant (p≤ 0. 05 – 0.001) analgesic effects that were comparable with the prototype drug, acetyl salicylic acid. This study validates its numerous applications in oedema and pain diseases in folkloric medicine.
Keywords: Protea madiensis, anti-inflammatory, analgesic, extract, fractions
Email of correspondence: uwemedimoumoh@uniuyo.edu.ng;
https://doi.org/10.59493/ajopred/2025.1.5 ISSN: 0794-800X (print); 1596-2431 (online)