ANTAGONISTIC EFFECT OF FOUR LACTOBACILLUS SP. ON MULTIDRUG RESISTANT KLEBSIELLA SP. GF01 IN COCULTURE
Adeoshun FG, Ayeni FA*
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
Afr. J Pharm Res Dev; Volume 8(2): 81-87; Nov/Dec 2016
ABSTRACT
The global antibiotic resistance in uropathogens has resulted in a search for alternatives to antibiotics. Lactic acid bacteria have a long history of safe use in the fight against various infectious states. This study is aimed at determining the antimicrobial activities of 4 species of lactobacilli previously isolated from Nigeria women during different stages of menstrual cycle against multidrug resistant uropathogenic Klebsiella sp. GF01 in coculture.
Four LAB has been previously identified from Nigerian women by sequencing the 16S rRNA gene. The antimicrobial properties of the LAB were tested against multidrug resistant uropathogenic strain of Klebsiella sp. GF01 using coculturing. Klebsiella sp. GF01 was freshly and at 8 hours growth, introduced into overnight growth of each lactobacilli, incubated for 24 hours, after which bacterial count of the uropathogen and Lactobacillus strains were performed In both series of experiment, Lactobacillus brevis GF021, Lactobacillus fermentum GF002 and Lactobacillus plantarum GF011 were active on Klebsiella sp. GF01 with 6log10 reduction (1010cfu/ml-104cfu/ml) after 24 h, while Lactobacillus fermentum GF019 show a low activity on Klebsiella sp. GF01 with 2log10 and 4log10 reduction (1010cfu/ml-108cfu/ml and 1010 cfu/ml-106cfu/ml) after 24 h for the first and second series of experiment respectively. It was observed that Klebsiella sp. GF01 do not have significant effect on any of the LAB strains. The presence of LAB was capable of inhibiting the growth of Klebsiella sp. GF01 freshly introduced and when it has already grown for 8h. The decrease in the number of Klebsiella sp. GF01 reveals the antagonistic effect of LAB cells on multidrug resistant Klebsiella sp. GF01.
*Corresponding Author: Fa.ayeni@ui.edu.ng