ANTIBACTERIAL ACTIVITY OF AGRICULTURAL WASTE-BASED ACTIVATED CARBONS AND SILVER-IMPREGNATED ACTIVATED CARBON AGAINST PATHOGENIC STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA
Nuhu AA1, Omali ICP2*, Lamis M3
1Department of Chemistry, Faculty of Physical Science, Ahmadu Bello University, Nigeria
2Department of chemistry, Faculty of Physical Science, Ahmadu Bello University, Nigeria
3Department of Chemistry, Faculty of Science, Gombe State University, Nigeria
Afr. J Pharm Res Dev; Volume 9(2): 133-140; Nov/Dec 2017
ABSTRACT
The ability of activated carbons from agricultural waste based precursors, Melaleuca leucadendron Husk (MLH), Caesalpinia pulcherrima husk (CPH), blended activated carbon (BAC-PA) and silver impregnated blended activated carbon (SIBAC-PA) to eliminate and destroy Staphylococcus aureus and Pseudomonas aeruginosa were tested under Plate Assay and Shake Flask techniques. SIBAC-PA showed the highest antibacterial effect against the two test organisms with inhibition zone diameters of 24 and 27 mm respectively. On using the shake flask technique, it was found that bacterial count started reducing after one hour incubation, while no bacterial growth was detected after 2,3 and 24 hours using the blended activated carbon. Bacterial growth was completely inhibited on using silver impregnated activated carbon at all the tested concentrations (12.5 – 50 mg/ml) after one hour incubation.
KEYWORDS: Activated carbon, Staphylococcus aureus, Pseudomonas aeruginosa, Agricultural waste
Corresponding author: omalipaul@gmail.com