ANTIDIABETIC ACTIVITY OF ISOLATES FROM THE LEAVES OF SECAMONE AFZELII RHOME (ASCLEPIADACEAE)
Abere TA*, Onwukaeme DN, Ozolua RI
1Department of Pharmacognosy, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Afr. J Pharm Res Dev; Volume 9(2): 119-124; Nov/Dec 2017
ABSTRACT
Secamone afzelii Rhome is used in ethnomedicine for diabetes and other disease conditions. This study was aimed at evaluating the antidiabetic activity of the aqueous ethanol extract and its isolates. Chromatographic techniques were employed in the isolation. Doses of the extract ranging from 100 to 500 mg/kg were evaluated in normoglycaemic rats. The dose of 500 mg/kg (crude extract and solvent fractions), 100 mg/kg (VLC fractions) and 50 mg/kg (isolated compounds) were evaluated in Streptozotocin-induced diabetic rats. Glibenclamide 1.25 mg/kg was used as the reference standard. The various doses of the crude extract produced significant (p <0.05) reduction in blood glucose levels in normoglycaemic and streptozotocin-induced diabetic rats. These doses were more effective than glibenclamide in normoglycaemic rats. Solvent partitioning, Vacuum Liquid Chromatography and Column Chromatographic analysis of extract of S. afzelii resulted in the isolation of two compounds which produced significant hypoglycaemic effects. S. afzelii and its isolated constituents possess antidiabetic properties in rats and are good candidates for further investigations.
KEYWORDS: Antidiabetic, Asclepiadaceae, Chromatography, Secamone afzelii
Corresponding author: eseabere@yahoo.com;