ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF Pseudomonas aeruginosa ISOLATED FROM CONTACT LENSES OF SOME SELECTED INDIVIDUALS IN CALEB UNIVERSITY, IMOTA, LAGOS STATE
OKUNYE OLUFEMI LIONEL1, *, IDOWU PHILIP ADEGBOYEGA2 , KOTUN BUNMI COMFORT3 AND KOLADE TITILAYO TENIOLA4
1. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Olabisi Onabanjo University, Ogun State.
2. Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan Nigeria.
3. Department of Biological Sciences and Biotechnology, Caleb University, Imota Lagos.
4. Centre for Entrepreneurship Development, Yaba College of Technology, Yaba Lagos.
Afr. J Pharm Res Dev; Volume 11(2): 151-155 ; 2019
Contact lenses are ocular prosthetic devices used by over 150 million people worldwide, and they can be worn to correct vision, for cosmetic, or therapeutic reasons. This epidemiological study was performed to determine the prevalence of Pseudomonas aeruginosa on contact lenses of selected students in Caleb University, Imota, Lagos State. A swab of 100 contact lenses from selected volunteers comprising 43 males and 57 females were collected for microbiological examination. Sterile swab sticks moistened with sterile distilled water were used to aseptically swab the lenses and transferred into nutrient broth followed by incubation for 5 hours. Thereafter, the resultant broth culture was subcultured on to cetrimide nutrient agar and incubated at 370C for 24 hours for the isolation of Pseudomonas aeruginosa. A total of 30 (30%) of the contact lenses sampled were found to have Pseudomonas aeruginosa, of which 13(43.3%) of the isolates were pigmented and 17( 56.7%) were non-pigmented. Eighty percent (80%) of the isolates were susceptible to ciprofloxacin, 75% to gentamicin while 50% were susceptible to ofloxacin. The isolates were 90% resistant to nitrofurantoin, 75% resistant to augmentin and 100% to ampicillin. Ciprofloxacin and gentamicin were found to be effective against Pseudomonas aeruginosa in this study and could be of therapeutic relevance for the management of contact lenses mediated infections.
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KEYWORDS: Antimicrobial susceptibility; Prevalence; Contact lenses; Pseudomonas aeruginosa