ANTINOCICEPTIVE AND ANTI-INFLAMMATORY ACTIVITIES OF ETHYLACETATE FRACTION OF CISSUS CORNIFOLIA PLANCH
Aliyu M*, Yaro AH, Muhammad M
Department of Pharmacology and Therapeutics, Faculty of Pharmaceutical Sciences, Bayero University Kano, Nigeria
Afr. J Pharm Res Dev; Volume 9(2): 175-181; Nov/Dec 2017
ABSTRACT
Cissus cornifolia (Vitaceae) is used traditionally in the treatment of malaria, septic tonsils, gonorrhea and other diseases. The analgesic and anti-inflammatory activities of crude methanol extract of Cissus cornifolia leaf had earlier been investigated. In this study, the antinociceptive, anti-inflammatory and antioxidant properties of ethylacetate fraction of crude methanol leaf extract was evaluated using the abdominal writhing, hot plate, carrageenan induced edema and DPPH radical scavenging tests. Doses used were 15, 30 and 60 mg/kg body weight in mice and rats. The ethylacetate fraction produced significant (P < 0.05- P < 0.001) reduction of number of writhes in the acetic acid induced test in mice. Thermal nociception induced by hot plate was significantly (P < 0.05) inhibited at the dose of 30 mg/kg of the fraction, and the effect that was blocked by naloxone. The ethylacetate fraction also showed significant (P < 0.001) anti-inflammatory effect in the carrageenan induced edema test. The DPPH radical scavenging activity was greater than that of ascorbic acid. These findings suggest that the ethylacetate fraction of C. cornifolia leaf possesses antinociceptive and anti-inflammatory activities which might have been enhanced by its antioxidant property.
KEYWORDS: Cissus cornifolia, Carrageenan, Hot plate, Pain, Inflammation, writhing
Corresponding author: maliyu.pha@buk.edu.ng;