ANTIOXIDANT AND AMELIORATIVE EFFECTS OF SINGLE DAILY DOSE OF AQUEOUS
EXTRACT OF Azanza garckeana ON FORMALIN-INDUCED TESTICULAR TOXICITY IN MALE
RATS
BUKATA B. BUKAR* , OMOLOLA D.G. ORSHI AND LERITSHIMWA AMAGON
Department of Pharmacology and Toxicology, University of Jos, P.M.B. 2048, Jos, Plateau state, Nigeria.
Afr. J Pharm Res Dev; Volume 13(1): 133-143 (2021)
ABSTRACT
The objective of this study was to evaluate the antioxidant and ameliorative effects of the aqueous extract of
Azanza garckeana on formalin-induced testicular toxicity in rats. Acute toxicity and phytochemical analyses of the
extract were conducted using standard procedures. Reproductive parameters and antioxidant enzymes were
determined using standard methods. The acute toxicity test showed that the LD50 of the extract is greater than
5,000 mg/kg, while the phytochemical analyses revealed the presence of abundant flavonoids with moderate
presence of alkaloids and tannins. Semen analyses showed a significant increase concentration at 125 mg/kg
compared to normal and formalin groups (p < 0.05). There was an increase in testosterone level at 125 mg/kg
compared to formalin group (p < 0.05). The antioxidant analyses revealed a significant increase activity of SOD at
125 mg/kg, and GSH and catalase at 250 mg/kg in formalin-induced toxicity compared to normal control (p < 0.05).
On the other hand, there was a decrease in concentration of MDA at 125 mg/kg. In conclusion, this investigation
revealed the anti-oxidant and ameliorative effects of Azanza garckeana that may serve as scientific justifications
of its ethno-medicinal use in male infertility
Email of correspondence: bukata33@yahoo.com
KEYWORDS: Azanza garckeana; Testosterone; Antioxidant; Gonadosomatic index; Infertility; Toxicity.