DEVELOPMENT OF GELATIN-SODIUM ALGINATE MICROPARTICLES FOR ORAL INSULIN DELIVERY

DEVELOPMENT OF GELATIN-SODIUM ALGINATE MICROPARTICLES FOR ORAL INSULIN DELIVERY

BEN CHIBUZOR AMADI1, PAUL AKPA2, EDUARDO BUXADERAS3,4, YANINA MOGLIE3,4,5, JOHN ALFA6, OMEH ROMANUS7, NNAMANI NNABUIKE DIDACUS8, FRANKLIN CHIMAOBI KENECHUKWU2, MOMOH AUDU MUMUNI2,6*, CÉSAR SALDÍAS9, DAVID DÍAZ DÍAZ3,5*

  1. Department of Pharmaceutical Technology and Industrial Pharmacy, University of Nigeria, Nsukka, Enugu State, Nigeria.
  2. Department of Pharmaceutics, Drug Delivery Research Unit, University of Nigeria, Nsukka, Enugu State, Nigeria.
  3. Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez 2, 38206 La Laguna, Spain.
  4. Instituto de Química del Sur, INQUISUR (CONICET-UNS), Departamento de Química, Universidad Nacional del Sur, Av. Alem 1253, 8000 Bahía Blanca, Argentina.
  5. Departamento de Química Orgánica, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez s/n, 38206 La Laguna, Spain.
  6. Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Bingham University Karu, Nasarawa State, Nigeria.
  7. Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Enugu State, Nigeria.
  8. Department of Pharmaceutics and Pharmaceutical Technology, Dora Akunyili College of Pharmacy Igbinedion University Okada, Edo State, Nigeria.
  9. Departamento de Química Física, Facultad de Química y Farmacia, Pontificia Universidad Católica de Chile, Macul, 7820436 Santiago, Chile.

Afr. J Pharm Res Dev; Volume 16(3): 105-116   ; 2024

ABSTRACT

Oral delivery of peptide drugs in the management of diabetes remained a major challenge among formulation scientists in the pharmaceutical industry. In this study, insulin-loaded microparticles for oral delivery were prepared with gelatin and sodium alginate and combined at different ratios using the double emulsion technique. The insulin-loaded microparticles (MPs) were evaluated for yield, and particle size, shape and thermal properties were determined. The in vitro release of insulin and blood glucose reduction after oral administration to diabetic rats were determined. The microparticles formed were spherical and pitted. The prepared insulin-loaded microparticles showed a lag phase before insulin release. The gelatin:sodium alginate (Na-Alg) ratio of 0:1 was the only formulation that released the most in vitro. The percentage blood glucose reduction for subcutaneously administered insulin was significantly greater than that for the formulation (p < 0.05). The reduction effect observed after orally administered insulin-loaded MPs in batch with Na-Alg-gelatin (1:1) was observed after 9 h. However, this effect was like the effect observed in subcutaneously insulin solution. There was gradual release with a sustain effect of lower blood glucose level for a period of 24 h. These results are indicative of its effectiveness as an alternative for the delivery of insulin.

 

Keywords: Insulin, Na-Alg, Diabetes, Gelatin

Email of correspondence: audu.momoh@unn.edu.ng,

https://doi.org/10.59493/ajopred/2024.3.12                                       ISSN: 0794-800X (print); 1596-2431 (online)

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