EFFECT OF EXTRACT AND FRACTIONS OF STEM BARK OF Morinda lucida BENTH (RUBICACEAE) ON CASTOR-OIL INDUCED DIARRHEA IN MICE
IBEABUCHI JUDE ALI1,*, OBIORA CELESTINE UGWU2, CYRIL CHEKWUBE ADONU3, ROMANUS CHIJIOKE OMEH4, FELIX KENOLISA ASOGWA2, NDIDIAMAKA HANNAH OKORIE1, RAYMOND MADUABUCHI OKONKWO2, ONYEKA OBIDIEGWU5, PATRICK CHIBUEZE ONYEGBULAM1, FESTUS BASDEN CHIEDU OKOYE5
- Department of Pharmaceutical Chemistry, Enugu State University of Science and Technology Agbani, Enugu State Nigeria
- Department of Pharmacology, Enugu State University of Science and Technology Agbani, Enugu State Nigeria
- Department of Pharmaceutical Microbiology and Biotechnology, Enugu State University of Science and Technology Agbani, Enugu State Nigeria
- Department of Pharmaceutics and Pharmaceutical Technology, Enugu State University of Science and Technology Agbani, Enugu State Nigeria.
- Department of Pharmaceutical and Medicinal Chemistry, Nnamdi Azikiwe University Awka, Anambra State Nigeria.
Afr. J Pharm Res Dev; Volume 16(3): 07-11 ; 2024
ABSTRACT
Morinda lucida Benth is a common Nigerian medicinal plant generally used to cure malaria parasite infection, though there is a lack of scientific reports on its capacity to treat diarrhea. The current study evaluated the effect of extract and fractions of stem bark of Morinda lucida Benth (Rubicaceae) on castor-oil-induced diarrhea in mice. The ground (500 g) was macerated in 2500 mL of methanol for 72 h, thereafter filtered and concentrated to obtain methanol extract. The extract (10 g) was subjected to gradient elution to afford different fractions. The phytochemical screening method was used to investigate the constituents of extract and fractions. The method of Lorke’s was employed in the acute toxicity study. The evaluation of anti-diarrhea activity was carried out by the castor-Oil-induced gastro-intestinal motility model. The secondary metabolites present in the extract and fractions were alkaloids, flavonoids, saponins, tannins, terpenoids, cardiac glycoside, anthraquinone glycoside, and steroids. The Acute toxicity indicated no mortality or any adverse behavioral change even at 5000 mg/kg body weight. The extract at 200 mg/kg dose gave better antidiarrheal activity than the 400 mg/kg dose indicating that the effect is not dose dependent. Among the fractions ethyl acetate at 400 mg/kg showed better activity and indicated no significant difference when compared to loperamide at 2 mg/kg dose. When compared, the antidiarrheal activity of the extract and fractions differed significantly at (p < 0.05) with negative control. Morinda lucida’s stem bark has an outstanding antidiarrheal effect as a result of its copious bioactive constituents.
Keywords: Anthraquinones, Morinda lucida, Castor-Oil, Diarrhea, Loperamide
Email of correspondence: ibeabuchi.ali@esut.edu.ng;
https://doi.org/10.59493/ajopred/2024.3.2 ISSN: 0794-800X (print); 1596-2431 (online)