IN-VITRO ANTI-INFLAMMATORY EVALUATION OF METHANOL AND BUTANOL EXTRACTS OF AVOACADO PEAR (PERSEA AMERICANA MILL) FRUIT PEEL
NDIDIAMAKA HANNAH OKORIE1,*, SUNDAY CHIDOZIE UMERIE2, EMEKA LEONIE CHISOM2, IBEABUCHI
JUDE ALI1
1. Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Enugu State University of Science and
Technology, Enugu State, Nigeria.
2. Department of Biochemistry, Faculty of Natural and Applied sciences. Paul University, Awka, Anambra State, Nigeria
Afr. J Pharm Res Dev; Volume 15(3): 01-06 ; 2023
ABSTRACT
Inflammatory diseases such as arthritis, rheumatoid or non-rheumatoid joint inflammations are dominant among people all around the world. Plant-derived anti-inflammatory drugs are widely used against these disease conditions. This study investigated the in-vitro anti-inflammatory activity of the aqueous methanol and butanol extracts of Persea americana fruit peel. Three different concentrations of test samples: 10, 20 and 40 mg/ml from the concentrated methanol extract (ME) and butanol extracts (BE) were evaluated in vitro by albumin denaturation, heat induced hemolysis, anti-proteinase action, anti-lipooxygenase spectrophotometric assay methods using aspirin as a reference drug. Results showed that at sample concentrations of 10, 20 and 40 mg/ml, the inhibition of albumin denaturation by the ME and BE were in the range 26.826-51.471 % and 40.329- 54.797 % respectively. The inhibition of heat-induced haemolysis by ME at the same concentrations was in the range 30.252-44.727% and 34.52-49.897% for BE. Anti-proteinase inhibitory activity for ME was within the range of 17.073-52.1 20% while 31 .781-37.01 4% was for BE. In all four assays, the BE elicited a relatively higher inhibitory activity than the ME. These suggest that the fruit extract possesses anti-inflammatory properties which may be attributed to the occurrence of bioactive compounds in the fruit peel.
Keywords: Persea americana, In-vitro, Anti-inflammatory, Spectrophotometry, Methanol extract, Butanol extract
Email of correspondence: hannah.okorie@esut.edu.ng;
https://doi.org/10.59493/ajopred/2023.3.1 ISSN: 0794-800X (print); 1596-2431 (online)