ISOLATION OF KAEMPFERIDE AND ANTIMICROBIAL ACTIVITY OF FRACTIONS OF AQUEOUS ETHANOL EXTRACT OF THESIUM VIRIDE

ISOLATION OF KAEMPFERIDE AND ANTIMICROBIAL ACTIVITY OF FRACTIONS OF AQUEOUS ETHANOL EXTRACT OF THESIUM VIRIDE

Shehu S¹*, Ibrahim G², Danmalam UH², October N³, Halilu ME¹, Abubakar MS²

1. Department of Pharmacognosy and Ethnopharmacy, Usmanu Danfodiyo University, Sokoto, Nigeria
2. Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria, Nigeria
3. Department of Chemistry, University of Pretoria, South Africa

Afr. J Pharm Res Dev; Volume 8(1): 19-23; June/July 2016

ABSTRACT

Kaempferide was isolated from the ethyl acetate fraction of the aqueous ethanol extract of Thesium viride (Santalacea), a plant used in traditional medicine for the treatment of jaundice and ulcer using a combination of silica gel column chromatography and preparative TLC. The structure of this compound was elucidated using NMR spectroscopic analysis and by comparison with reported data. This is the first report of isolation of this compound from this plant. The antimicrobial activity of n-hexane, chloroform, ethyl acetate and n-butanol fractions was determined on some gram negative, gram positive bacteria and fungi. The ethyl acetate fraction was found to have higher inhibitory effect to the test microorganisms.

*Corresponding Author: pharmsani@gmail.com