ISOLATION OF LUPEOL, OLEANYL ERUCOATE AND OLEANOLIC-3-ACETATE FROM
CRYPTOLEPIS OBLONGIFOLIA (MEISN) SCHLTR.
SAIFULLAHI UMAR1,*, ANAS ABBA1 , MSHELIA EMMANUEL HALILU2
- Department of Pharmacognosy and Herbal Medicine, Bayero University, Kano
- Department of Pharmacognosy and Ethnobotany, Usmanu Danfodiyo University, Sokoto
Afr. J Pharm Res Dev; Volume 15(1): 9-16; 2023
ABSTRACT
Cryptolepis oblongifolia (Periplocaceae) is a thin-stem twinning and scrambling shrub with height of 1 m and is locally used in the treatment of malaria, inflammation and stomach disorders. Column chromatography of the n-hexane and ethyl acetate extracts of C. oblongifolia led to the isolation of compounds A1, B1 and C1 which were identified using 1HNMR, 13CNMR, DEPT 135, HSQC and HMBC spectra. The melting point of compounds A1, B1 and C1 were found to be 175-176oC, 78-80oC and 216-217o C respectively. Red colouration was observed for the three compounds when tested with Lieberman-Burchard’s reagent which indicated the presence of triterpenoid nucleus. The 1HNMR of the compounds (A1, B1 and C1) revealed doublet of doublet around 3.5 ppm. The 13CNMR for A1 indicated unsaturation at 109 and 150 ppm (Lupane group), while B1 indicated saturated oleanane group and C1 indicated unsaturation around 122.0 and 144.0 ppm (Olean-12-ene). The chemistry of Lupeol, Oleanyl erucoate and Oleanolic -3- acetate will help greatly in understanding their pharmacological activities.
Email of correspondence: sumar.phc@buk.edu.ng;
KEYWORDS: Cryptolepis, Lupeol, Oleanolic -3-acetate, Oleanyl erucoate