rbcL GENE SEQUENCING AND RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) PROFILING OF Pavonia senegalensis (CAV.) LIESTNER (MALVACEAE) A MEDICINAL PLANT COLLECTED FROM GIWA NORTHWEST NIGERIA
SHEHU UMAR FARUK*, ABUBAKAR ABDULMUMIN ZAYD, ADAMU ABDULRAHMAN, IBRAHIM GARBA, ILYAS NAJMA
Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria.
Afr. J Pharm Res Dev; Volume 11(2): 088-094 ; 2019
ABSTRACT
Pavonia senegalensis (Cav.) Liestner is a medicinal plant used in African traditional medicine for the treatment of diarrhea, soft and tissue infections, induction of labor and contraception. In this study, ribulose-1, 5-biphosphate carboxylase (rbcL)gene sequence and random amplification of polymorphic DNA (RAPD) profile of the plant were determined. The study showed that the species of the plant is phylogenetically related to Abutilon sp., Hibiscus micranthus, Hibiscus punalunensis, Hibiscus rosa-sinensis and Lagunaria patersonia all from the same family showing 98% similarity on the basis of rbcL gene sequences. The studied specimen and related taxa inferred from partial rbcL gene sequence demonstrated a distinct lineage; therefore, could distinguish the species as P. senegalensis without doubt. RAPD profile of P. senegalensis generated amplified products ranging from 320 to 700 bp. A total of 9 bands were observed for the plant species using 3 primers (OP-A07, OP-A10 and OP-C06). The determined rbcL gene sequence and RAPD profile of the P. senegalensis will help in quality control for authentication and detection of adulteration in the development of the plant as an herbal medicine.
Email of correspondence:umarfarukshehu@gmail.com
KEYWORDS: DNA; Primers; Phylogenetic tree; PCR.