UTEROTONIC EFFECT OF METHANOL ROOT BARK EXTRACT OF ENTADA MANNII (OLIV.) TISSER
ADEBAYO ANTHONY GBOLADE1,*, SONIA KESIENA OGONA1
- Phytotherapy Research Group, Department of Pharmacognosy, College of Pharmacy, Igbinedion University Okada, Edo State, Nigeria.
Afr. J Pharm Res Dev; Volume 17(1): 9-17 ; 2025
ABSTRACT
Entada mannii (Oliv.) Tisser. (Fabaceae) has a range of uses in African traditional medicine such as treatment of respiratory ailments, gonorrhoea, yellow fever and toothache. This study is aimed at justifying folkloric usage of E. mannii in inducing labour, and it evaluated possible contractile effect of the crude methanol (MeOH) extract and aqueous (AQ) fraction of the root bark on isolated uterus of oestrogenised non-pregnant female rats. Spontaneous contraction model at 1.0 – 8.0 mg/mL extract, oxytocin-induced contraction model at 6.7 µg/mL in physiological salt solution (PSS), calcium ions-free PSS model, and high KCL concentration-induced contraction (80 mM) model were employed. Extract gave higher increases in amplitude (2-fold) and frequency (> 2 fold) of spontaneous uterine contraction than AQ fraction in a dose-dependent manner. For the oxytocin pre-contracted uteri in normal PSS and calcium ions free-PSS, as well as high KCL concentration-induced contraction, decreases in amplitude and frequency of contractions were recorded with both agents, suggesting the plant did not potentiate the effects of these agonists. This result, suggests smooth muscle uterotonic effect for E. mannii root bark.
Keywords: Entada mannii, Root bark, Uterotonic effect, Rat uterus, Methanol extract, Aqueous fraction
Email of correspondence: gbolade.adebayo@iuokada.edu.ng;
https://doi.org/10.59493/ajopred/2025.1.2 ISSN: 0794-800X (print); 1596-2431 (online)